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Image Search Results
Journal: PLoS ONE
Article Title: Forkhead box C2 Promoter Variant c.-512C>T Is Associated with Increased Susceptibility to Chronic Venous Diseases
doi: 10.1371/journal.pone.0090682
Figure Lengend Snippet: (A) Gel image showing increased expression of Dll4 and Hey2 mRNA in EA.hy926 cells transfected with FoxC2 –pCAGIG. Lane 1: 100 bp molecular ladder, Lane 2, 4, 6, 8: FoxC2, GAPDH, Dll4 and Hey2 mRNA levels in cells transfected with pGAGIG control vector. Lane 3, 5, 7, 9: FoxC2, GAPDH, Dll4 and Hey2 mRNA levels in FoxC2 - pGAGIG construct transfected cells. (B) Gel image showing increased expression of COUP TFII and Ephrin B4 mRNA in EA.hy926 cells transfected with FoxC2 –pCAGIG. Lane 1, 3, 5: Ephrin B4, COUP TFII and GAPDH mRNA levels in cells transfected with empty pGAGIG control vector. Lane 2, 4, 6: Ephrin B4, COUP TFII and GAPDH mRNA levels in FoxC2 - pGAGIG construct transfected cells, Lane 7: 100 bp molecular ladder (C) Relative mRNA levels of Hey2 and Dll4 measured by qRT-PCR. (D) Relative mRNA levels of COUP TFII and Ephrin B4 measured by qRT-PCR. Values are mean±s.d. of triplicate experiments from 3 samples per each group. Statistical significance was determined by Student's t-test (*p<0.05 versus control).
Article Snippet: FoxC2 – pCAGIG construct was made by inserting FoxC2 coding sequence into EcoRI and XhoI restriction sites of
Techniques: Expressing, Transfection, Control, Plasmid Preparation, Construct, Quantitative RT-PCR